Aqueous two-phase system purification for superoxide dismutase induced by menadione from Phanerochaete chrysosporium.
Identifieur interne : 000623 ( Main/Exploration ); précédent : 000622; suivant : 000624Aqueous two-phase system purification for superoxide dismutase induced by menadione from Phanerochaete chrysosporium.
Auteurs : Berna Kavakc O Lu [Turquie] ; Burcu Tongul [Turquie] ; Leman Tarhan [Turquie]Source :
- Artificial cells, nanomedicine, and biotechnology [ 2169-141X ] ; 2017.
Descripteurs français
- KwdFr :
- Ménadione (pharmacologie), Phanerochaete (enzymologie), Polymères (composition chimique), Polyéthylène glycols (composition chimique), Propylène glycols (composition chimique), Protéines fongiques (composition chimique), Protéines fongiques (isolement et purification), Superoxide dismutase (composition chimique), Superoxide dismutase (isolement et purification).
- MESH :
- composition chimique : Polymères, Polyéthylène glycols, Propylène glycols, Protéines fongiques, Superoxide dismutase.
- enzymologie : Phanerochaete.
- isolement et purification : Protéines fongiques, Superoxide dismutase.
- pharmacologie : Ménadione.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Fungal Proteins, Polyethylene Glycols, Polymers, Propylene Glycols, Superoxide Dismutase.
- chemical , isolation & purification : Fungal Proteins, Superoxide Dismutase.
- enzymology : Phanerochaete.
- chemical , pharmacology : Vitamin K 3.
Abstract
In the present work, the partitioning behavior of menadione-induced superoxide dismutase (SOD; EC 1.15.1.1), an antioxidant enzyme that has various applications in the medical and cosmetic industries, from the white rot fungus Phanerochaete chrysosporium has been characterized on different types of aqueous two-phase systems (ATPSs) (poly(ethylene glycol)/polypropylene glycol (PEG/PPG)-dextran, PEG-salt and PPG-salt). PEG-salt combinations were found most optimal systems for the purification of SOD. The best partition conditions were found using the PEG-3350 24% and K2HPO4 5% (w/w) with pH 7.0 at 25 °C. The partition coefficient of total SOD activity and total protein concentration observed in this system were 0.17 and 6.65, respectively, with the recovery percentage as 78.90% in the bottom phase and 13.17% in the top phase. The highest purification fold for SOD from P. chrysosporium was found as 6.04 in the bottom phase of PEG 3350%24 - K2HPO4%5 (w/w) system with pH 7.0. SOD purified from P. chrysosporium was determined to be a homodimer in its native state with a molecular weight of 60 ± 4 kDa. Consequently, simple and only one step PEG-salt ATPS system was developed for SOD purification from P. chrysosporium.
DOI: 10.3109/21691401.2016.1160404
PubMed: 27022668
Affiliations:
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</wicri:regionArea><wicri:noRegion>Izmir </wicri:noRegion></affiliation></author><author><name sortKey="Tongul, Burcu" sort="Tongul, Burcu" uniqKey="Tongul B" first="Burcu" last="Tongul">Burcu Tongul</name><affiliation wicri:level="1"><nlm:affiliation>a Department of Chemistry, Faculty of Science , University of Dokuz Eylul , Buca, Izmir , Turkey.</nlm:affiliation><country xml:lang="fr">Turquie</country><wicri:regionArea>a Department of Chemistry, Faculty of Science , University of Dokuz Eylul , Buca, Izmir </wicri:regionArea><wicri:noRegion>Izmir </wicri:noRegion></affiliation></author><author><name sortKey="Tarhan, Leman" sort="Tarhan, Leman" uniqKey="Tarhan L" first="Leman" last="Tarhan">Leman Tarhan</name><affiliation wicri:level="1"><nlm:affiliation>a Department of Chemistry, Faculty of Science , University of Dokuz Eylul , Buca, Izmir , Turkey.</nlm:affiliation><country xml:lang="fr">Turquie</country><wicri:regionArea>a Department of Chemistry, Faculty of Science , University of Dokuz Eylul , Buca, Izmir </wicri:regionArea><wicri:noRegion>Izmir </wicri:noRegion></affiliation></author></analytic><series><title level="j">Artificial cells, nanomedicine, and biotechnology</title><idno type="eISSN">2169-141X</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Fungal Proteins (chemistry)</term><term>Fungal Proteins (isolation & purification)</term><term>Phanerochaete (enzymology)</term><term>Polyethylene Glycols (chemistry)</term><term>Polymers (chemistry)</term><term>Propylene Glycols (chemistry)</term><term>Superoxide Dismutase (chemistry)</term><term>Superoxide Dismutase (isolation & purification)</term><term>Vitamin K 3 (pharmacology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Ménadione (pharmacologie)</term><term>Phanerochaete (enzymologie)</term><term>Polymères (composition chimique)</term><term>Polyéthylène glycols (composition chimique)</term><term>Propylène glycols (composition chimique)</term><term>Protéines fongiques (composition chimique)</term><term>Protéines fongiques (isolement et purification)</term><term>Superoxide dismutase (composition chimique)</term><term>Superoxide dismutase (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Fungal Proteins</term><term>Polyethylene Glycols</term><term>Polymers</term><term>Propylene Glycols</term><term>Superoxide Dismutase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Fungal Proteins</term><term>Superoxide Dismutase</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Polymères</term><term>Polyéthylène glycols</term><term>Propylène glycols</term><term>Protéines fongiques</term><term>Superoxide dismutase</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Protéines fongiques</term><term>Superoxide dismutase</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Ménadione</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Vitamin K 3</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In the present work, the partitioning behavior of menadione-induced superoxide dismutase (SOD; EC 1.15.1.1), an antioxidant enzyme that has various applications in the medical and cosmetic industries, from the white rot fungus Phanerochaete chrysosporium has been characterized on different types of aqueous two-phase systems (ATPSs) (poly(ethylene glycol)/polypropylene glycol (PEG/PPG)-dextran, PEG-salt and PPG-salt). PEG-salt combinations were found most optimal systems for the purification of SOD. The best partition conditions were found using the PEG-3350 24% and K<sub>2</sub>HPO<sub>4</sub> 5% (w/w) with pH 7.0 at 25 °C. The partition coefficient of total SOD activity and total protein concentration observed in this system were 0.17 and 6.65, respectively, with the recovery percentage as 78.90% in the bottom phase and 13.17% in the top phase. The highest purification fold for SOD from P. chrysosporium was found as 6.04 in the bottom phase of PEG 3350%24 - K<sub>2</sub>HPO<sub>4</sub>%5 (w/w) system with pH 7.0. SOD purified from P. chrysosporium was determined to be a homodimer in its native state with a molecular weight of 60 ± 4 kDa. Consequently, simple and only one step PEG-salt ATPS system was developed for SOD purification from P. chrysosporium.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27022668</PMID><DateCompleted><Year>2017</Year><Month>01</Month><Day>30</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">2169-141X</ISSN><JournalIssue CitedMedium="Internet"><Volume>45</Volume><Issue>2</Issue><PubDate><Year>2017</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Artificial cells, nanomedicine, and biotechnology</Title><ISOAbbreviation>Artif Cells Nanomed Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Aqueous two-phase system purification for superoxide dismutase induced by menadione from Phanerochaete chrysosporium.</ArticleTitle><Pagination><MedlinePgn>380-388</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3109/21691401.2016.1160404</ELocationID><Abstract><AbstractText>In the present work, the partitioning behavior of menadione-induced superoxide dismutase (SOD; EC 1.15.1.1), an antioxidant enzyme that has various applications in the medical and cosmetic industries, from the white rot fungus Phanerochaete chrysosporium has been characterized on different types of aqueous two-phase systems (ATPSs) (poly(ethylene glycol)/polypropylene glycol (PEG/PPG)-dextran, PEG-salt and PPG-salt). PEG-salt combinations were found most optimal systems for the purification of SOD. The best partition conditions were found using the PEG-3350 24% and K<sub>2</sub>HPO<sub>4</sub> 5% (w/w) with pH 7.0 at 25 °C. The partition coefficient of total SOD activity and total protein concentration observed in this system were 0.17 and 6.65, respectively, with the recovery percentage as 78.90% in the bottom phase and 13.17% in the top phase. The highest purification fold for SOD from P. chrysosporium was found as 6.04 in the bottom phase of PEG 3350%24 - K<sub>2</sub>HPO<sub>4</sub>%5 (w/w) system with pH 7.0. SOD purified from P. chrysosporium was determined to be a homodimer in its native state with a molecular weight of 60 ± 4 kDa. Consequently, simple and only one step PEG-salt ATPS system was developed for SOD purification from P. chrysosporium.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kavakcıoğlu</LastName><ForeName>Berna</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>a Department of Chemistry, Faculty of Science , University of Dokuz Eylul , Buca, Izmir , Turkey.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tongul</LastName><ForeName>Burcu</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>a Department of Chemistry, Faculty of Science , University of Dokuz Eylul , Buca, Izmir , Turkey.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tarhan</LastName><ForeName>Leman</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>a Department of Chemistry, Faculty of Science , University of Dokuz Eylul , Buca, Izmir , Turkey.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>03</Month><Day>29</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Artif Cells Nanomed Biotechnol</MedlineTA><NlmUniqueID>101594777</NlmUniqueID><ISSNLinking>2169-1401</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011108">Polymers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011409">Propylene Glycols</NameOfSubstance></Chemical><Chemical><RegistryNumber>25322-69-4</RegistryNumber><NameOfSubstance UI="C012504">polypropylene glycol</NameOfSubstance></Chemical><Chemical><RegistryNumber>3WJQ0SDW1A</RegistryNumber><NameOfSubstance UI="D011092">Polyethylene Glycols</NameOfSubstance></Chemical><Chemical><RegistryNumber>723JX6CXY5</RegistryNumber><NameOfSubstance UI="D024483">Vitamin K 3</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.15.1.1</RegistryNumber><NameOfSubstance UI="D013482">Superoxide Dismutase</NameOfSubstance></Chemical><Chemical><RegistryNumber>U076Q6Q621</RegistryNumber><NameOfSubstance UI="C000595209">polyethylene glycol 1000</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011092" MajorTopicYN="N">Polyethylene Glycols</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011108" MajorTopicYN="N">Polymers</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011409" MajorTopicYN="N">Propylene Glycols</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013482" MajorTopicYN="N">Superoxide Dismutase</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D024483" MajorTopicYN="N">Vitamin K 3</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Aqueous two-phase systems</Keyword><Keyword MajorTopicYN="N">partitioning</Keyword><Keyword MajorTopicYN="N">polymer/salt system</Keyword><Keyword MajorTopicYN="N">purification</Keyword><Keyword MajorTopicYN="N">superoxide dismutase</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>3</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>1</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>3</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27022668</ArticleId><ArticleId IdType="doi">10.3109/21691401.2016.1160404</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Turquie</li></country></list><tree><country name="Turquie"><noRegion><name sortKey="Kavakc O Lu, Berna" sort="Kavakc O Lu, Berna" uniqKey="Kavakc O Lu B" first="Berna" last="Kavakc O Lu">Berna Kavakc O Lu</name></noRegion><name sortKey="Tarhan, Leman" sort="Tarhan, Leman" uniqKey="Tarhan L" first="Leman" last="Tarhan">Leman Tarhan</name><name sortKey="Tongul, Burcu" sort="Tongul, Burcu" uniqKey="Tongul B" first="Burcu" last="Tongul">Burcu Tongul</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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